Unpurified PCR Template Sequencing Service
Many commercial PCR purification products are not robust enough for DNA sequencing purpose. One of biggest challenges is that the recovery yield is quite variable with these commercial kits. Laragen has developed a proprietary PCR purification technique to overcome this low and uneven yield issue. This service is similar to the purified template sequencing service except we will purify PCR DNA for you.
With this service, customers will perform PCR reactions in 25 ul volume. Run 5 ul on an agarose gel. If there is a single band in the PCR product, send us the rest of the 20 ul PCR DNA. It will help us to have a gel image picture along with the samples. No purification is needed. Laragen will perform PCR purification. We also require 5 ul of 10 uM primer for each sequencing reaction. Please don’t label the PCR strips on the caps. Please label on the side of the tubes.
The turnaround time is overnight. Average reads can be up to 900 bp depending on DNA quality and DNA and primer sequences. We will provide a free repeat for failed reaction for this service.
For 96-well plate, please arrange samples by the columns, not by the rows. You can use upload and import file functions in our DNALIMS system to upload 96-well plate format. No spaces or illegal characters allowed in the DNALIMS. Allowable characters include: a - z, A - Z, 0 - 9, and hyphens. You can use this template for file upload.
Please seal the 96-well plate with caps, foil tape or heat seal tape to avoid possible cross contamination and sample evaporation. Please label on the side of plate. Don’t label on the top (seal or caps) of the plates.
If you aren't using our convenient free pickup service, ship your samples at room temperature to:
10601 Virginia Ave.
Culver City, CA 90232