RCA Sequencing (Sequencing from Bacterial Cells)
We will use the RCA technique to amplify the circular plasmids DNA from bacterial cells and sequence the amplified DNA directly. This technique is especially good for low copy number plasmids and will save you time to prepare the miniprep.
Turnaround time for RCA service is within 48 hours.
Sample Requirements:
1) Low density cell culture: This is the most popular format. We accept 500 ul of 2x LB overnight culture at 37oC with shaking at 300 rpm in deep well culture blocks. The culture block should be sealed with airpore tape. For the shipping purpose, we recommend you send the cell pellets by centrifuging and decanting the culture medium. The pellets need to be sent on dry ice to keep frozen.
2) Glycerol stock: 50 to 100 ul of glycerol stocks can be sent frozen on dry ice in 96-well plates. Seal plate with foil tape or caps.
3) Bacterial colonies on agar plate: The colonies on the agar plate need to be well separated to enable us to pick a single colony. If you need the colonies back, please number the colonies so you can match the sequencing results with the colonies. Wrap the plates with parafilm.
For 96-well plate, please arrange samples by the columns, not by the rows: You can use upload and import file function in our DNALIMS system to upload 96-well plate format. No spaces or illegal characters allowed in the DNALIMS. Allowable characters include: a - z, A - Z, 0 - 9, and hyphens. You can use this template for file upload.
Please seal the 96-well plate with caps, foil tape or heat seal tape to avoid possible cross contamination and sample evaporation. Please label on the side of plate. Don’t label on the top (seal or caps) of the plates.
If you aren't using our convenient free pickup service, ship your samples at room temperature to:
Laragen, Inc.
10601 Virginia Ave.
Culver City, CA 90232
Testimonial
"We use Laragen’s sequencing service to provide sequences from microsatellite-enriched libraries. It’s important that the sequences be accurate because we inspect the scans to find microsatellites, and use them to design PCR primers around the microsatellites we find. It’s also important that the sequences be returned to us quickly so that we can pass the results on to our customers. We have been very satisfied on all counts: the scans are robust, making microsatellites easy to spot, 90% or more of the primers are successful, and we get the sequences back rapidly, always within 24 hours. We are very pleased with Laragen’s service."
Ken Jones, President/CEO, Genetic Identification Services
News
Laragen 2010 Holiday Schedule --- Laragen to close on Nov 25 and 26 in observance of Thanksgiving and ... more news...
Laragen Moving Date ---
Nov 27, 2010 Laragen is moving to 10601 Virginia Ave...more news...