Laragen Sequencing Genotyping

Premix Sequencing Service

Premix sequencing service is designed for customers who want to save sequencing cost and do not want to set up their own sequencing reactions.

Sample Requirements:

With this services, customers will measure the DNA concentration and mix 200 ng of plasmid DNA (4 to 6 kb, or more DNA if the plasmids are large) and 10 to 50 ng of PCR DNA (100 to 50 bp, 10 ng for every 100 bp of PCR fragment) with 10 pmole of sequencing primer in a total of 6 ul in 8 strip PCR tubes. Please use sequencing grade water. Please don’t label the PCR strips on the caps. Please label on the side of the tubes.

The turnaround time is overnight. Average reads can be up to 900 bp depending on DNA quality and DNA and primer sequences.

The key issue to having long sequencing reads is the quality and quantity of DNA. Too much or too little DNA and dirty DNA are the main causes of the reaction failure. We recommend Qiagen miniprep kits to prepare plasmid DNA. Certain commercial kits did not work well with automated Sanger sequencing. EDTA will inhibit DNA sequencing reaction. Don’t elute DNA into TE buffer. Use only water or Qiagen EB buffer (10 mM TrisHCl, pH 8.0).

For 96-well plate, please arrange samples by the columns, not by the rows: You can use upload and import file function in our DNALIMS system to upload 96-well plate format. No spaces or illegal characters allowed in the DNALIMS. Allowable characters include: a - z, A - Z, 0 - 9, and hyphens. You can use this template for file upload.

Please seal the 96-well plate with caps, foil tape or heat seal tape to avoid possible cross contamination and sample evaporation. Please label on the side of plate. Don’t label on the top (seal or caps) of the plates.

If you aren't using our convenient free pickup service, ship your samples at room temperature to:

Laragen, Inc.
10601 Virginia Ave.
Culver City, CA 90232