Probe Based TaqMan PCR Mouse Genotyping
Most of Laragen’s mouse genotyping tests are probe-based TaqMan assays. Laragen’s TaqMan assays have an internal positive control on a housekeeping gene to ensure the test integrity. Besides all tests also have CAR (HOMO and HET, if applicable), WT and NTC controls. The samples in the grey zone will be repeated multiple times to ensure test accuracy.
The turnaround time for probe based mouse genotyping is 24 to 48 hours.
We require 0.3 to 0.4 cm tail clips and 2 mm ear punches. It is important that the tissue samples have similar sizes. Samples can be sent in either 1.7 ml Eppendorf tubes or 1.1ml 8-strip microtubes in 96 well boxes. Samples in 96-well plate format should be arranged by the columns as indicated below.
Mouse tails should be send on dry ice and DNA samples can be sent at room temperature.