Heterozyogsity Mouse Genotyping by TaqMan
Laragen also provides heterozygosity tests for transgenic mice based on qPCR (TaqMan) via copy number analysis. Normalized high quality genomic DNA samples are tested in triplicates and the transgene copy number of unknown samples are compared with the known het and homo controls.
The turnaround time for heterozygosity testing is 24 to 48 hours.
Sample Requirements:
We require 0.3 to 0.4 cm tail clips and 2 mm ear punches. It is important that the tissue samples have similar sizes. Samples can be sent in either 1.7 ml Eppendorf tubes or 1.1ml 8-strip microtubes in 96 well boxes. Samples in 96-well plate format should be arranged by the columns as indicated below.
Mouse tails should be send on dry ice and DNA samples can be sent at room temperature.
Please send a hard copy of Laragen's Mouse Genotyping Service Request Form with your samples and email an electronic version to mousegenotyping@laragen.com.
Testimonial
"We have been using the Laragen genotyping service for the last 5 years. We
found that they provided very consistent service and great turnaround time."
Sylvia Chen, Ph.D. Buck Institute
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