Gel Based PCR Mouse Genotyping
A small portion of Largen’s mouse genotyping tests are gel based PCR assays. Like probe-based assays, we also include an internal positive control PCR on a house-keeping gene to ensure PCR integrity. All PCR reactions are optimized to yield accurate genotyping results even at suboptimal conditions. A vigorous SOP is implemented to eliminate PCR contamination problems.
The turnaround time is 24 to 48 hours.
Sample Requirements:
We require 0.3 to 0.4 cm tail clips and 2 mm ear punches. It is important that the tissue samples are uniform in size. Samples can be sent in either 1.7 ml Eppendorf tubes or 1.1 ml 8-strip microtubes in 96 well boxes. Samples in 96-well plate format should be arranged by columns as indicated below.
Mouse tails should be send on dry ice and DNA samples can be sent at room temperature.
Please send a hard copy of Laragen's Mouse Genotyping Service Request Form with your samples and email an electronic version to mousegenotyping@laragen.com.
Testimonial
"We are very pleased with the results from Laragen. Our research certainly has been sped up because of your services. We are happy to receive high quality genotyping services at such a reasonable rate."
Eisuke Koya, NIH
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