Human Cell Line Authentication-Mycoplasma Detection
Mycoplasma testing is carried out using two methods at the same time point. Detecting mycoplasma by PCR amplification is highly sensitive and is recommended by The American Type Culture Collection (ATCC.) All contaminated cultures should be treated or discarded. Mycoplasma belongs to the family Mollicutes; including Mycoplasma, Acholeplasma, Ureaplasma, Entomoplasma, and Spiroplasma. They are the smallest free-living and self-replicating organisms. Six Mycoplasma species: M.orale, M.arginii, M.fermentans, M.salivarum, M.hyorhinis and A.laidlawii, account for 95% of all mycoplasma contamination in cell cultures.
- Extract genomic DNA cell pellets and/or FTA paper
- Perform both general Mycoplasma specific PCR and Mycoplasma species specific PCR
- The PCR products were then sequenced and Mycoplasma species was identified based on our and the Genebank database