Sanger Sequencing

Laragen has been providing the highest quality sequencing services to both academic institutions and private companies. Laragen utilizes an ABI Prism 3730 DNA sequencer so you can be confident that you are getting the best data possible for your genomics projects every time. Our success is based on a variety of strengths ranging from our high quality Long Sequencing read (up to 1100 bp), to our convenient free sample pickup, to our competitive rates and bulk discounts. In addition, we offer friendly, effective customer support and the ability to handle difficult templates (GC rich and RNAi templates and bisulfite sequencing) making us your best choice for sequencing services.

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Mouse Genotyping

Laragen has been providing transgenic, knock-out, knock-in and shRNA transgenic mouse genotyping services since 2002. Most of our mouse genotyping services are performed with probe based qPCR (TaqMan Assay). Our goal is to provide fast and accurate mouse genotyping services to researchers from both academic and industrial institutions. Unlike most of our competitors, who use crude tail lysate in the tests, Laragen uses high quality genomic DNA extracted with our proprietary DNA extraction technique. High quality genomic DNA is one of the key factors that helps us obtain above 99.95% accuracy in mouse genotyping.

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MiSeq Sequencing

Laragen began offering MiSeq NexGen sequencing service in 2013. In MiSeq, cluster generation, sequencing, and data analysis are all done on a single instrument. MiSeq read length is much longer than HiSeq, which make it perfect for metagenomic studies. With MiSeq V3 2 x 300 kit, the MiSeq can produce 25 million of 600 bp reads. With MiSeq V2 2 x 250 kit, the MiSeq can produce 15 million of 500 bp reads.

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Cell Line ID

Laragen provides reliable, fast, and affordable STR based human cell line authentication services. Our STR cell line authentication tests comply with ANSI/ATCC ASN-0002-2011 guidelines. STR (Short Tandem Repeat) has been used in human paternity and forensic fields for more than 20 years. In recent years, STR-based analysis has been used as the “golden standard” in human cell line authentication. Laragen has over 10 years experience working on STR PCR and fragment analysis.

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Fragment Analysis

Laragen provides fast and reliable fragment analysis services with an AB3730XL. We have three standard dye sets available from Applied Biosystems: DS-30 (6-FAM, VIC, NED and ROX), DS-31 (6-FAM, VIC, NED and ROX) and DS-33 (6-FAM, VIC, NED PET and LIZ). ROX and LIZ are used for internal size standards. We will choose different size standards based on your needs. Our experienced team will help you to design ready to run fragment analysis projects.

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Microbial ID

Based on the length of rDNA used, Laragen currently provides two tests of bacterial identification by 16S rDNA and one test of fungal identification by 28S rDNA. We use Genebank databases to identify the unknown bacteria and fungi. The advantages of bacterial and fungal identification by rDNA sequences are fast turnaround time and accurate results even for problematic bacteria and fungi. All reports include the consensus sequence of the tested bacterial/fungal sequences, the alignment of the top 10 matches at the species or genus level and a phylogenetic tree with these top 10 sequences.

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Laragen, Inc
10601 Virginia Ave, Culver City, CA90232
310-280-0804 info@laragen.com